Single-Pollen Genotyping by Yuji Isagi (auth.), Yuji Isagi Ph.D, Yoshihisa Suyama Ph.D

By Yuji Isagi (auth.), Yuji Isagi Ph.D, Yoshihisa Suyama Ph.D (eds.)

Pollination is likely one of the most vital procedures in plant replica. It without delay affects reproductive luck and health and the genetic constitution of the plant inhabitants. tools exist to deduce the trend and distance of pollen dispersal, yet direct commentary of the hobbies of person pollen grains in the course of pollination isn't possible due to their small measurement. Single-pollen genotyping is a unique process for genotyping a unmarried pollen grain. during this booklet, the foundations, the experimental protocol, and several other purposes of this technique in stories of plant ecology, reproductive biology, and evolutionary genetics were defined. extra particularly, the data turns out to be useful for the research of linkage disequilibrium, intraspecific genetic version, chromosome mapping, and the origins of polyploidy. it's also crucial for attaining sustainable and optimum crop yield and is key to agriculture and forestry. Written via pioneer researchers, the ebook presents novel learn methods which are proving worthy in progressively more fields. This quantity is meant to inspire new and endured functions of single-pollen genotypingamongmany disciplines sooner or later.

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Plant Species Biol 5:255–261 Latouchee-Hallé C, Ramboer A, Bandou E, Caron H, Kremer A (2004) Long-distance pollen flow and tolerance to selfing in a neotropical tree species. Mol Ecol 13:1055–1064 Lloyd DG (1992) Self- and cross-fertilization in plants. II. The selection of self-fertilization. Int J Plant Sci 153:370–380 Marshall TC, Slate J, Kruuk LEB, Pembertom M (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655 Matsuki Y, Isagi Y, Suyama Y (2007) The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain.

Beetles primarily pollinate plant species that retain primitive characteristics (Thien et al. 2000). The Magnoliaceae is a well-known example; beetle pollination syndrome in the genus Magnolia was recognized in the late 1800s. However, in the flowers of Magnolia obovata, a common Magnolia species in Japan, it is often observed that not only beetles but also bees, flies, and some thrips visit the flowers. How do these flower-visiting insects contribute to reproduction in M. obovata? We focused on the pollination system of M.

Mol Ecol 12:753–764 Diels L (1916) Kaferblumen bei den Ranales und ihre Bedeutung für die Phylogenese der Angiospermen. Ber Dtsch Bot Ges 34:758–774 Dieringer G, Cabrera RL, Lara M, Loya L, Reyes-Castillo P (1999) Beetle pollination and floral thermogenicity in Magnolia tamaulipana (Magnoliaceae). Int J Plant Sci 160:64–71 Dramstad WE (1996) Do bumblebees (Hymenoptera: Apidae) really forage close to their nests? J Insect Behav 9:163–182 Elliott SE, Irwin RE (2009) Effects of flowering plant density on pollinator visitation, pollen receipt, and seed production in Delphinium barbeyi (Ranunculaceae).

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